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Student Number 985201014
Author Yung-nian Wei(魏永年)
Author's Email Address No Public.
Statistics This thesis had been viewed 867 times. Download 11 times.
Department Electrical Engineering
Year 2010
Semester 2
Degree Master
Type of Document Master's Thesis
Language zh-TW.Big5 Chinese
Title Glycated Hemoglobin Detection with Screen-printed Carbon Electrode by AC Impedance Analysis
Date of Defense 2011-07-04
Page Count 159
Keyword
  • AC impedance analysis
  • electrode
  • glycated hemoglobin
  • hemoglobin
  • screen-printing
  • Abstract This research focused on hemoglobin (Hb) and glycated hemoglobin (HbA1c) detection using disposable carbon electrodes and AC impedance analysis. The disposable carbon electrodes were fabricated by screen-printing technology. Among the three fabricated electrode configurations, the one that led to the largest relative impedance difference was used in the measurement. The natures of Hb and HbA1c are different. They have diffreent amount of charged particles in the solution. By AC impedance analysis, we can obtain stable and significant change in the solution resistance in the equivalent circuit model. By this way we knew the concentration range of Hb and HbA1c corresponding to the maximum detection sensitivity. A self-made circuit was used in the impedance measutement. A microcontroller was responsible for measurement control and data transmission. Measurement data were saved and processed with MATLAB. The HbA1c measurement results were similar with the self-made citcuit and with the commercail impedance analyser, which is expensive and not portable. The self-made citcuit and disposable carbon electrodes have made the measurement easy, fast, inexpensive, and portable.
    Table of Content 摘要I
    ABSTRACTII
    致謝III
    目錄IV
    圖目錄VII
    表目錄XVI
    第一章 前言1
    1-1 糖尿病1
    1-1-1 糖尿病簡介1
    1-1-2糖尿病的檢測方法6
    1-2文獻回顧9
    1-2-1生物感測器簡介9
    1-2-2網版印刷生物感測器12
    1-2-3電化學感測器量測方法22
    1-2-4網印技術於生物感測應用28
    第二章 研究動機與目的32
    2-1研究動機32
    2-2研究目標33
    第三章 實驗方法34
    3-1 網版印刷電極34
    3-1-1 商業電極34
    3-1-2自製網版印刷電極36
    3-2 電極分析方法50
    3-2-1儀器設備50
    3-2-2電極電化學特性阻抗量測54
    3-2-3不同溶液阻抗量測55
    3-3 蛋白質實驗步驟方法56
    3-3-1 血紅素阻抗量測56
    3-3-2不同濃度血紅素阻抗量測57
    3-3-2不同濃度糖化血紅素阻抗量測57
    3-3-4糖化血紅素不同百分比含量阻抗量測58
    3-4 蛋白質阻抗實驗方法59
    3-4-1阻抗量測59
    3-4-2 自製量測系統60
    第四章 結果與討論75
    4-1網版印刷電極製程結果75
    4-1-1商業電極75
    4-1-2自製電極82
    4-1-3電極比較89
    4-2網版印刷電極量測結果92
    4-2-1商業電極特性95
    4-2-2自製網版印刷電極特性98
    4-2-3三種電極比較101
    4-3蛋白質阻抗量測結果106
    4-3-1血紅素量測106
    4-3-2不同濃度血紅素量測116
    4-3-3不同濃度糖化血紅素量測119
    4-3-4糖化血紅素不同百分比含量阻抗量測122
    4-4自製量測系統125
    4-4-1電路實現125
    4-4-2量測結果127
    第五章 結論129
    未來展望132
    參考文獻133
    Reference [1]Prevention of blindness from diabetes mellitus, Report of WHO consulation in Geneva, Switzerland (2005), 9-11
    [2]Homas R Moore, Diabetes Mellitus and Pregnancy. eMedicine. Version: Jan 27, (2005) 
    [3]Abdhish Bhavsar, Diabetic Retinopathy, 來源Medscape, 2010年12月21日取自http://emedicine.medscape.com/article/1225122-overview
    [4]Definition and diagnosis of diabetes mellitus and intermediate hyperglycemia,World Health organization (2006)
    [5]Sean F. Dinneen, Robert A. Rizza, Progression From Newly Acquired Impaired Fasting Glusose to Type 2 Diabetes, Diabetes Care. 30(2) (2007) 228-33.
    [6]Huisman T.H., Martis E.A., Dozy A. Chromatography of hemoglobin types on carboxymethylcellulose. J. Lab. Clin. Med.52 (2): 312–27
    [7]Rahbar S, Blumenfeld O, Ranney HM . "Studies of an unusual hemoglobin in patients with diabetes mellitus".Biochem. Biophys. Res. Commun. 36 (5): 838–43
    [8]Diabetes Control and Complications Trial Research Group: The effect of intensive diabetes treatment on the development and progression of long-term complications in insulin-dependent diabetes mellitus: Diabetes Control and Complications Trial. N Engl J Med (1993) 329:978–986
    [9]Limin Liu, Stefanie Hood, Yuping Wang, Robert Bezverkov, Chao Dou, Abhijit Datta, Chong Yuan, Direct enzymatic assay for %HbA1c in human whole blood samples, Clinical Biochemistry 41 (2008) 576 – 583
    [10]David E. Goldstein,  Randie R. Little,  Rodney A. Lorenz,  John I. Malone,  David Nathan,  Charles M. Peterson, David B. Sacks,  Tests of Glycemia in Diabetes, Diabetes Care July 2004 vol. 27 no. 71761-1773, American Diabetes Association
    [11]Seung Yeon Song, Hyun C. Yoon, Boronic acid-modified thin film interface for specific binding of glycated hemoglobin (HbA1c) and electrochemical biosensing, Sensors and Actuators B 140 (2009) 233–239
    [12]D.M. Nathan, J. Kuenen, R. Borg, H. Zheng, D. Schoenfeld, R.J. Heine, Translating the A1C Assay Into Estimated Average Glucose Values, Diabetes Care 31 (2008) 1473–1478
    [13]Serena Laschi, Marco Mascini, Planar electrochemical sensors for biomedical applications, Medical Engineering & Physics 28 (2006) 934–943
    [14]孔版印刷的發展, 來源:中華印刷通史, 2010年1月10日取自http://www.cgan.net/book/books/print/g-history/gb_9/23_7.htm
    [15]平面網印機產品圖, 來源:東遠精技股份有限公司,  2011年2月15日取自http://www.atma.com.tw/
    [16]平網曲面機產品圖, 來源:Skyhill明太網印儀器公司, 2011年2月15日取自http://www.ming-tai.com.tw/e-catalog/index.html
    [17]圓網印刷機產品圖, 來源:DGE德高機電企業集團2011年2月15日取自http://www.xadegao.com/jingtai.asp
    [18]Pablo Fanjul-Bolado, David Hern´andez-Santos, Pedro Jos´e Lamas-Ardisana Alberto Mart´ın-Pern´ıa,  Agust´ın Costa-Garc´ıa, Electrochemical characterization of screen-printed and conventional carbon paste electrodes, Electrochimica Acta 53 (2008) 3635–364
    [19]E. Khaled, Gehad G. Mohamed, T. Awad, Disposal screen-printed carbon paste electrodes for the potentiometric titration of surfactants, Sensors and Actuators B 135 (2008) 74–80
    [20]J. Wang, B. Tian,V.B. Nascimento, L. Angnes, Performance of screen –printed carbon electrodes fabricated from different carbon inks, Electrochimica Acta, Vol. 43, No. 23, (1998) 3459-3465
    [21]D.D. Gornall, S.D. Collyer, Séamus P.J. Higson, Investigations into the use of screen-printed carbon electrodes as templates for electrochemical sensors and sonochemically fabricated microelectrode arrays, Sensors and Actuators B 141 (2009) 581–591
    [22]F. Ghamouss, E. Luais, C. Thobie-Gautier, P.-Y. Tessier, M. Boujtita, Argon plasma treatment to enhance the electrochemical reactivity of screen-printed carbon surfaces, Electrochimica Acta 54 (2009) 3026–3032
    [23]H. Wei, J.J. Sun, Yu Xie, C.G. Lin,Y.M. Wang, W.H. Yin, G.N. Chen, Enhanced electrochemical performance at screen-printed carbon electrodes by a new pretreating procedure, Analytica Chimica Acta 588 (2007) 297–303
    [24]M. Pravda, C.O’Meara, G.G. Guilbault, Polishing of screen-printed electrodes improves IgG adsorption, Talanta 54 (2001) 887–892
    [25]S.C. Wang, K.S. Chang, C.J. Yuan, Enhancement of electrochemical properties of screen-printed carbon electrodes by oxygen plasma treatment, Electrochimica Acta 54 (2009) 4937–4943
    [26]M.D. Osborne , B.J. Seddon , R.A.W. Dryfe , G. Lagger , U. Loyal1 , H. Schifer , H.H. Girault , Excimer laser-induced electrochemical activity in carbon ink films, Journal of Electroanalytical Chemistry 417 (1996) 5-15
    [27]W.Y. Su, S.M. Wang, S.H. Cheng, Electrochemically pretreated screen-printed carbon electrodes for the simultaneous determination of aminophenol isomers, Journal of Electroanalytical Chemistry 651 (2011) 166–172
    [28]F. Ghamouss, P.-Y. Tessier, M.A. Djouadi, M.-P. Besland, M. Boujtita, Examination of the electrochemical reactivity of screen printed carbon electrode treated by radio-frequency argon plasma, Electrochemistry Communications 9 (2007) 1798–1804
    [29]E. Crouch, D.C. Cowell, S. Hoskins, R.W. Pittson, J. P. Hart, A novel disposable, screen-printed amperometric biosensor for glucose in serum fabricated using a water-based carbon ink, Biosensors and Bioelectronics 21 (2005) 712–718
    [30]Pedro José Lamas-Ardisana, Alberto Martín-Pernía and Agustín Costa-García, Pablo Fanjul-Bolado, David Hernández-Santos, Electrochemical characterization of screen-printed and conventional carbon paste electrodes, Electrochimica Acta 53 (2008) 3635–3642
    [31]F. Ghamouss, P.-Y. Tessier, A. Djouadi, M.-P. Besland, M. Boujtita, Screen-printed carbon electrode modified on its surface with amorphous carbon nitride thin film: Electrochemical and morphological study, Electrochimica Acta 52 (2007) 5053–5061
    [32]吳浩青, 李永舫, 電化學動力學, 科技圖書出版社, 2001
    [33]P. Tamiasso-Martinhon, H. Cachet, C. Debiemme-Chouvy, C. Deslouis, Thin films of amorphous nitrogenated carbon a-CNx, Electrochimica Acta 53 (2008) 5752–5759
    [34]S. Hleli, C. Martelet,, A. Abdelghani, F. Bessueille, A. Errachid, J. Samitier,H.C.W. Hays, P.A. Millner, N. Burais, N. Jaffrezic-Renault, An immunosensor for haemoglobin based on impedimetric properties of a new mixed self-assembled monolayer, Materials Science and Engineering C 26 (2006) 322 – 327
    [35]T. Balkenhohl, F. Lisdat, Screen-printed electrodes as impedimetric immunosensors for the detection of anti-transglutaminase antibodies in human sera, Analytica Chimica Acta 597 (2007) 50–57
    [36]Cesar Fernandez-Sanchez, Calum J. McNeil, Keith Rawson, Electrochemical impedance spectroscopy studies of polymer degradation: application to biosensor development, Trends in Analytical Chemistry, Vol. 24, No. 1, 2005
    [37]David A. Harrington, P. van den Driessche, Mechanism and equivalent circuits in electrochemical impedance spectroscopy, Electrochimica Acta (2011) 
    [38]CMS300 Electrochemical System Operator’s Manual, Gamery Instruments, Inc., USA, 1993.
    [39]Ivanildo Luiz de Mattos, Lo Gorton , Tautgirdas Ruzgas, Sensor and biosensor based on Prussian Blue modified gold and platinum screen printed electrodes, Biosensors and Bioelectronics 18 (2003) 193-200
    [40]Gehad G. Mohamed, Tamer Awad Ali, M.F. El-Shahat, A.M. Al-Sabagh,M.A. Migahed, Elmorsy KhaledD, Potentiometric determination of cetylpyridinium chloride using a new type of screen-printed ion selective electrodes, Analytica Chimica Acta 673 (2010) 79–87
    [41]J.P. Hart, A.K. Abass, D. Cowell, Development of disposable amperometric sulfur dioxide biosensors based on screen printed electrodes, Biosensors & Bioelectronics 17 (2002) 389–394
    [42]K.C. Honeychurch, J.P. Hart, P.R.J. Pritchard, S.J. Hawkins, N.M. Ratcliffe, Development of an electrochemical assay for 2,6-dinitrotoluene, based on a screen-printed carbon electrode, and its potential application in bioanalysis, occupational and public health, Biosensors and Bioelectronics 19 (2003) 305-312
    [43]Belen Bello Rodriguez, John A. Bolbot, Ibtisam E. Tothill, Development of urease and glutamic dehydrogenase amperometric assay for heavy metals screening in polluted samples, Biosensors and Bioelectronics 19 (2004) 1157–1167
    [44]L. Micheli, R. Grecco, M. Badea, D. Moscone, G. Palleschi, An electrochemical immunosensor for aflatoxin M1 determination in milk using screen-printed electrodes, Biosensors and Bioelectronics 21 (2005) 588–596
    [45]Jie Wu, Jinhai Tang, Zong Dai, Feng Yan, Huangxian Ju, Nabil El Murr, A disposable electrochemical immunosensor for flow injection immunoassay of carcinoembryonic antigen, Biosensors and Bioelectronics 22 (2006) 102–108
    [46]Guido Carpini, Fausto Lucarelli, Giovanna Marrazza, Marco Mascin, Oligonucleotide-modified screen-printed gold electrodes for enzyme-amplified sensing of nucleic acids, Biosensors and Bioelectronics 20 (2004) 167–175
    [47]Lam Dai Tran, Binh Hai Nguyen, Nguyen Van Hieu, Hoang Vinh Tran,Huy Le Nguyen, Phuc Xuan Nguyen, Electrochemical detection of short HIV sequences on chitosan/Fe3O4 nanoparticle based screen printed electrodes, Materials Science and Engineering C 31 (2011) 477–485
    [48]W. Laureyn, D. Nelis, P. Van Gerwen, K. Baert, L. Hermans, R. Magnee, J.-J. Pireaux, G. Maes, Nanoscaled interdigitated titanium electrodes for impedimetric biosensing, Sensors and Actuators B 68 (2000) 360-370
    [49]T. Houssin, J. Follet, A. Follet, E. Dei-Cas, V. Senez, Label-free analysis of water-polluting parasite by electrochemical impedance spectroscopy, Biosensors and Bioelectronics 25 (2010) 1122–1129
    [50]P.V. Gerwen, W. Laureyn, W. Laureys, G. Huyberechts, Maaike Op De Beeck, Kris Baert, Jan Suls, Willy Sansen, P. Jacobs, Lou Hermans, Robert Mertens, Nanoscaled interdigitated electrode arrays for biochemical sensors, Sensors and Actuators B 49 (1998) 73–80
    [51]Enrique Valera, Javier Ram´on-Azc´on, ´Angel Rodr´ıguez, Luis M. Casta˜ner, F.-J. S´anchez, M.-P. Marco, Impedimetric immunosensor for atrazine detection using interdigitated μ-electrodes (IDμE’s), Sensors and Actuators B 125 (2007) 526–537
    [52]W. Laureyn, D. Nelis, P. Van Gerwen, K. Baert, L. Hermans, R. Magnee , J.-J. Pireaux, G. Maes, Nanoscaled interdigitated titanium electrodes for impedimetric biosensing, Sensors and Actuators B 68 2000 360–370
    [53]Angel Rodr´ıguez, Enrique Valera, Javier Ramon-Azc´on´, F.-J. Sanchez, M.-P. Marco, Luis M. Castaner, Single frequency impedimetric immunosensor for atrazine detection, Sensors and Actuators B 129 (2008) 921–928
    [54]Enrique Valera, David Muñiz, Ángel Rodríguez, Fabrication of flexible interdigitated l-electrodes (FIDlEs) for the development of a conductimetric immunosensor for atrazine detection based on antibodies labelled with gold nanoparticles, Microelectronic Engineering 87 (2010) 167–173
    [55]Analog Devices AD5933data sheet, Analog Devices
    [56]A Technical Tutorialon Digital Signal Synthesis data sheet, Analog Devices
    [57]戴佳, 戴衛恆, 8051單晶片C語言應用程式設計實力詳解, 松岡電腦圖書有限公司, 2007
    [58]鄭錦聰, 莊鎮家, MATLAB程式設計實務, 全華圖書股份有限公司, 2009
    [59]Attila Vig, Xavier Mu noz-Berbel, Antonio Radoi, Montserrat Cortina-Puig, Jean-Louis Marty, Characterization of the gold-catalyzed deposition of silver on graphite screen-printed electrodes and their application to the development of impedimetric immunosensors, Talanta 80 (2009) 942–946
    [60]Hong Cai, Thomas Ming-Hung Lee, I-Ming Hsing, Label-free protein recognition using an aptamer-based impedance measurement assay, Sensors and Actuators B 114 (2006) 433–437
    [61]Jung A Lee, Seongpil Hwang, Juhyoun Kwak, Se Il Park, Seung S. Lee, Kwang-Cheol Lee, An electrochemical impedance biosensor with aptamer-modified pyrolyzed carbon electrode for label-free protein detection, Sensors and Actuators B 129 (2008) 372–379
    [62]Zhiwei Zou, Junhai Kai, Michael J. Rust, Jungyoup Han, Chong H. Ahn, Functionalized nano interdigitated electrodes arrays on polymer with integrated microfluidics for direct bio-affinity sensing using impedimetric measurement, Sensors and Actuators A 136 (2007) 518–526
    [63]Haiying Yu, HermannBerg, Electrofusion of protoplasts modified by protein adsorption, Bioelectrochemistry and Bioenergetics 44 1998 233–236
    [64]Electrochemical detection of HbA1c, a maker for diabetes,using a flow immunoassay system, Tsuyoshi Tanaka , Shoko Tsukube , Kojiro Izawa , Mina Okochi , Tae-Kyu Limb, Shugo Watanabe , Manabu Harada , Tadashi Matsunaga, Biosensors and Bioelectronics 22 (2007) 2051–2056
    [65]J. Wang, Analytical Electrochemistry 3rd edition, John Wiley & Sons, 2006
    [66]Phosphate Buffer Saline E404-100TABS技術資料, aMResco
    [67]ITK碳漿SC-1010 技術資料, 愛迪克科技股份有限公司
    [68]Carbon Graphite Ink GWENT-C2050517D1技術資料, Gwent Group
    [69]Carbon Graphite Ink GWENT-C2030519P4技術資料, Gwent Group
    [70]ZAHNER IM6-ex電化學量測儀操作手冊, ZAHNER
    [71]HIOKI 3532-50 LCR HiTESTER阻抗分析儀操作手冊, HIOKI
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  • Jang-Zern Tsai(蔡章仁)
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    Date of Submission 2011-08-24

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