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Student Number 953203094
Author Da-Shiang Ma(馬大翔)
Author's Email Address No Public.
Statistics This thesis had been viewed 1300 times. Download 686 times.
Department Mechanical Engineering
Year 2008
Semester 1
Degree Master
Type of Document Master's Thesis
Language zh-TW.Big5 Chinese
Title Study of the Effects of Shear Stresses on the Growth and Morphology of Rat Bone Marrow Stem Cells with the Cone-Plate Bioreactor
Date of Defense 2008-01-08
Page Count 72
Keyword
  • Bioreactor
  • Cone-Plate Bioreactor
  • Shear Stress
  • Stem Cells
  • Tissue engineering
  • Abstract We designed a cone-plate bioreactor. It produces fluid shear stress by rotating the cone that drives the culture medium to flow in a circular way. Rat bone marrow stem cells were cultured on coverslips placed on the plate. The cells experienced the shear stress of 1.4、5.5、11、16.4 dyn/cm2 respectively for 4 hours in 1 day and 14 hours in the bioreactor.
    Results showed the shear stresses changed the morphology and inhibited the cell growth when the shear magnitudes were over 1.4 dyn/cm2. We also found that, by applying shear stress of 1.4 dyn/cm2 for 4 hours within 3 days and 14 hours, the cells became elongated in shape and aligned to each other.
    The study demonstrates that the cone-plate bioreactor can provide well-defined stimulus to the cells cultured in vitro. The growth inhibition and shape changes of the cells by shears shall merit further research efforts to classify the underlying mechanisms and biochemical pathways.
    Table of Content 中文摘要………………………… І
    英文摘要…………………………II
    誌謝………………………………Ⅲ
    目錄………………………………IV
    圖目錄………………………. …VII
    第一章 緒論
    1.1 前言 ………………………………………………………1
    1.2 文獻回顧……………………………………………………4
    1.2.1 生物反應器與剪應力刺激機械刺激……………………4
    1.2.2 剪應力生物反應器………………………………………7
    1.3 研究動機……………………………………………………9
    第二章 生物反應器設計與製作
    2.1 設計前言…………………………………………………………………10
    2.2 剪應力產生原理以及剪應力計算…………………………10
    2.2.1 剪應力產生原理…………………………………………10
    2.2.2 定常剪應力計算…………………………………………11
    2.3 培養室設計…………………………………………………12
    2.3.1 圓錐平板尺寸設計………………………………………12
    2.3.2 系統剪應力性能表現……………………………………14
    2.4 生物反應器機構設計………………………………………15
    2.4.1 培養環境系統……………………………………………17
    2.4.2 馬達驅動裝置與馬達箱體………………………………18
    2.4.3 承載平板空間……………………………………………25
    2.4.4 圓錐平板間距定位調整…………………………………26
    第三章 實驗方法
    3.1 細胞來源……………………………………………………29
    3.2 玻片滅菌程序………………………………………………29
    3.3 細胞種植……………………………………………………31
    3.4 實驗前置準備………………………………………………31
    3.5 生物反應器系統培養效能與細胞相容性測試……………33
    3.6 實驗步驟……………………………………………………36
    3.7 系統操作步驟………………………………………………38
    3.8 實驗條件……………………………………………………39
    3.9 馬達轉速設定………………………………………………40
    3.10 結果分析…………………………………………………40
    3.10.1 細胞型態觀察…………………………………………41
    3.10.2 細胞數量計數…………………………………………41
    第四章 結果與討論
    4.1 剪應力強度對細胞生長狀態的影響………………………43
    4.1.1 剪應力刺激強度:1.4 dyn/cm2............................................44
    4.1.2 剪應力刺激強度:5.5 dyn/cm2............................................46
    4.1.3 剪應力刺激強度:11 dyn/cm2............................................49
    4.1.4 剪應力刺激強度:16.4 dyn/cm2............................................51
    4.2 剪應力對細胞型態的… …………………………………54
    第五章 結論與未來展望………………………………………63
    參考文獻..…………………………………………………………………65
    附錄
    實驗試藥及儀器………………………………………………72
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    Advisor
  • Chih-Ang Chung(鍾志昂)
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    Date of Submission 2009-02-02

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