Title page for 943203114


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Student Number 943203114
Author Yung-Chou Yeh(葉永州)
Author's Email Address kogate4556677@yahoo.com.tw
Statistics This thesis had been viewed 1675 times. Download 0 times.
Department Mechanical Engineering
Year 2006
Semester 2
Degree Master
Type of Document Master's Thesis
Language zh-TW.Big5 Chinese
Title Development of A Manual liquid nitrogen Speciment storage system
Date of Defense 2007-07-07
Page Count 96
Keyword
  • brittle fracture at low-temperature
  • stem cells
  • temperature effect
  • umbilical cord blood
  • Abstract The progress of biotechnology not merely advents the human welfare, but reveals great economic potential. With the development of technology and the attraction of business profits, the biotechnology is destined to become a nouveau high-tech industry where the whole world will pour into enormous efforts.
    This study aims to develop a container with liquid nitrogen at -196°C as a more suitable storage for slice of organism, by extracting advantage and eliminating limitation from currently-adopted containers. At present, there are the traditional manual lid-opening container by Taylor-Wharton, the mechanical container by Cryo-Cell (Cryo-Cell International Inc., USA), the one by BioArchive (Thermogenesis Corp., USA) and others in Taiwan. 
      The study procedure consists of two parts: container’s manufacture and software development. The former is achieved by the following steps: (1) draw the container model with 3D software; (2) calculate the structural strength with the finite-element analysis; (3) examine whether the stress concentration exceeds the material strength of the container; (4) manufacture the container and conduct the wear tests. The latter is to develop window-style software to control and monitor the operation and function of the container.
      The results of the current study demonstrate the practicability of the new-designed container. Using this container, the operator could easily and conveniently put into and take off a single cartridge without any power supply, thus preventing the other cartridges in the same container from a decrease in the biological activity. Moreover, the development of the user-friendly software can facilitate and enhance the practicability and convenience of the hardware and software.
    Table of Content 中文摘要 ……………………………………………………………  i
    英文摘要……………………………………………………………  ii
    誌謝……………………………………………………………  iv
    目錄……………………………………………………………  v
    圖目錄…………………………………………………………… viii
    表目錄…………………………………………………………… xii
    一、緒論………………………………………………………   1
    1-1研究背景…………………………………………………   1
    1-2研究動機與目的…………………………………………   3
    1-3液態氮超低溫生物試片儲存槽介紹……………………    4
    1-3-1傳統液態氮超低溫生物試片儲存槽……………………   4
    1-3-2機械式自動化液態氮超低溫生物試片儲存槽…………   7
    1-3-3傳統與機械自動化液態氮超低溫生物試片儲存槽比較………...   10
    1-3-4新型液態氮超低溫生物片儲存槽………………………  14
    1-4文獻回顧…………………………………………………  15
    1-4-1溫度傷害效應……………………………………………  15
    1-4-2低溫不鏽鋼轉脆效應……………………………………  17
    1-4-3安全警示系統……………………………………………  19
    1-4-4人機介面…………………………………………………  20
    1-5本文架構…………………………………………………  24
    二、材料與方法………………………………………………  25
    2-1超低溫生物試片儲存槽設計理念與開發流程…………  26
    2-1-1傳動機構的設計原理……………………………………  26
    2-1-2感測警戒裝置的安排……………………………………  27
    2-1-3人機介面環境的規劃……………………………………  28
    2-2超低溫生物試片儲存槽電腦實體模型之建立…………  31
    2-2-1儲存槽零件的設計………………………………………  31
    2-2-2儲存槽系統的組合………………………………………  41
    2-3超低溫生物試片儲存槽電腦模擬分析…………………  45
    2 - 3 -1有限元素運算參數的設定……………………………………..    46
    2 - 3 -2分析參數與變形準則……………………………………….…   49
    2-4超低溫生物試片儲存槽之製作…………………………  50
    2-5超低溫生物試片儲存磨耗測試…………………………  55
    三、結果………………………………………………………  59
    3 -1     有限元素分析結果……………………………………………..   59
    3 -2    超低溫生物試片儲存槽之製作結果……………………  61
    3 -3    磨耗測試結果……………………………………………  62
    四、討論………………………………………………………  65
    4 -1     有限元素分析結果討論……………………………………….     65
    4 -2    超低溫生物試片儲存槽之製作結果討論………………  67
    4 -3    磨耗測試結果討論………………………………………  70
    五、結論與未來展望…………………………………………  74
    5-1結論………………………………………………………  74
    5-2未來展望…………………………………………………  75
    參考文獻…………………………………………………………...   76
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    Advisor
  • Shang-Chih Lin(林上智)
  • Files
  • 943203114.pdf
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    Date of Submission 2007-07-17

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