Title page for 92223038


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Student Number 92223038
Author Pi-Lien Wang(王碧蓮)
Author's Email Address No Public.
Statistics This thesis had been viewed 2509 times. Download 11 times.
Department Chemistry
Year 2004
Semester 2
Degree Master
Type of Document Master's Thesis
Language zh-TW.Big5 Chinese
Title Determination of pharmaceutical resides in waters by solid phase extraction with liquid chromatography-tandem mass spectrometry
Date of Defense 2005-06-10
Page Count 106
Keyword
  • carbamazepine
  • clofibric acid
  • diclofenac
  • ibuprofen
  • ketoprofen
  • LC-MS/MS
  • naproxen
  • solid phase extraction
  • Abstract A method for the determination of selected pharmaceutical residues (clofibric acid, ibuprofen, carbamazepine, naproxen, ketoprofen and diclofenac) in various water samples was developed and validated. The contents of pharmaceutical residues in water samples were extracted by Oasis HLB solid-phase extraction (SPE). The analytes were then identified and quantitated by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Three ionization techniques, electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI), were tested, while ESI in negative and positive modes (for carbamazepine) proved to be the most sensitive ionization method. Limits of quantitation (LOQ) were established between 2.0 to 200 ng/L in 250 mL of water sample. Intrabatch and interbatch precision with their accuracy at two concentration levels were also investigated. The selected analytes were detected in concentrations from 24 to 963 ng/L in wastewater treatment plant effluent and river water samples.
    Table of Content 目錄
    中文摘要 ------------------------------------------------------------------------Ⅰ
    英文摘要 ------------------------------------------------------------------------Ⅱ
    謝誌 ------------------------------------------------------------------------------Ⅲ
    目錄 ------------------------------------------------------------------------------Ⅳ
    表目錄 ---------------------------------------------------------------------------Ⅶ
    圖目錄 ---------------------------------------------------------------------------Ⅸ
    第一章 前言 ---------------------------------------------------------------------1
    1-1研究源起 ------------------------------------------------------------------1
    1-2研究目標 ------------------------------------------------------------------4
    第二章 文獻回顧 ---------------------------------------------------------------6
    2-1 藥物殘留物的來源 -----------------------------------------------------6
    2-2 藥物殘留物在環境中的作用機制與影響 --------------------------6
    2-3 分析物與相關文獻 -----------------------------------------------------8
    2-3-1 分析物 ------------------------------------------------------------8
    2-3-2 國外相關文獻 -------------------------------------------------10
    2-4 固相萃取法 -------------------------------------------------------------16
    2-5 液相層析質譜儀 -------------------------------------------------------19
    2-5-1 電噴灑游離法 -------------------------------------------------20
    2-5-2 大氣壓力化學游離法 ----------------------------------------22
    2-5-3 大氣壓力光游離法 -------------------------------------------22
    第三章 實驗步驟與樣品分析 -----------------------------------------------24
     3-1 實驗藥品與儀器設備 -------------------------------------------------24
       3-1-1 實驗藥品 -------------------------------------------------------24
       3-1-2 儀器設備 -------------------------------------------------------26
     3-2實驗步驟 -----------------------------------------------------------------27
       3-2-1 標準品的製備 -------------------------------------------------27
       3-2-2 標準品的測定 -------------------------------------------------28
       3-2-3 萃取步驟 -------------------------------------------------------31
       3-2-4 真實水樣萃取及淨化 ----------------------------------------32
    第四章 結果與討論 -----------------------------------------------------------33
    4-1 不同游離源在正負離子模式下之分析結果-----------------------33
    4-2 液相層析儀之沖提流速對於不同離子源的影響-----------------38
    4-2-1不同的沖提流速對APCI游離源之影響--------------------40
    4-2-2不同的沖提流速對APPI游離源之影響--------------------42
    4-2-3不同的沖提流速對ESI游離源之影響----------------------47
     4-3 檢量線--------------------------------------------------------------------57
    4-4 固相萃取-----------------------------------------------------------------59
     4-5 真實水樣之分析結果--------------------------------------------------61
    第五章 結論---------------------------------------------------------------------86
    參考文獻--------------------------------------------------------------------------88
    Reference 參考文獻
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    Advisor
  • Wang-Hsien Ding(丁望賢)
  • Files
  • 92223038.pdf
  • disapprove authorization
    Date of Submission 2005-06-20

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