Title page for 89321046


[Back to Results | New Search]

Student Number 89321046
Author Ming-Tsang Wang(王明滄)
Author's Email Address s9321046@cc.ncu.edu.tw
Statistics This thesis had been viewed 2069 times. Download 2231 times.
Department Chemical and Materials Engineering
Year 2001
Semester 2
Degree Master
Type of Document Master's Thesis
Language zh-TW.Big5 Chinese
Title Effect of ORP on hydrogen production by clostridium butyricum ia bubble column bioreactor
Date of Defense 2002-07-13
Page Count 56
Keyword
  • hydrogen
  • oxidation reduction potential
  • Abstract Hydrogen is considered to be one of the best alternative fuels to petroleum. Most of pollutants of our environment are resulted from widely using petroleum as fuels. Hydrogen is also the cleanest energy because its product is water after burning with oxygen. It is believed that hydrogen will replace the petroleum as the major fuel in the next era.
    Table of Content 目錄
    摘要……………………………………………………………. Ⅰ
    目錄……………………………………………………………. Ⅲ
    圖索引…………………………………………………………..Ⅴ
    表索引………………………………………………………….Ⅶ
    第一章 緖論……………………………………………………1
    1-1研究動機…………………………………………………1
    1-2研究目的…………………………………………………2
    第二章 文獻回顧………………………………………………3
    2-1前言………………………………………………………3
    2-2燃料電池簡介……………………………………………5
    2-3 微生物產氫簡介………….………………………………7
    2-4 Clostridium…………………………………………………9
    2-5 厭氧微生物產氫機制……………………………………12
    2-6 環境因子對菌種產氫的影響……………………………18
    2-7 氧化還原電位……………………………………………22
    第三章 材料與方法……………………………………………26
    3-1 實驗材料…………………………………….……………26
    3-1-1 微生物……………………………………………………26
    3-1-2 培養基組成………………………………………………26
    3-1-3 實驗藥品…………………………………………………26
    3-1-4 實驗儀器與設備…………………………………………27
    3-2 實驗方法…………………………………………….……29
    3-2-1菌種保存…………………………………………………30
    3-2-2接種菌體培養……………………………………………30
    3-2-3厭氧微生物反應器………………………………………30
    3-2-4生長曲線的測定…………………………………………32
    3-2-5氣體分析…………………………………………………32
    3-2-6葡萄糖的分析……………………………………………33
    3-2-7代謝酸的分析……………………………………………34
    3-3  有機酸對C. butyricum產氫的影響…………………….35
    3-4  pH值對C. butyricum產氫的影響………………………35
    3-5  連續式饋料產氫……………………..…………………...35
    第四章 結果與討論……………………………………………..36
    4-1有機酸對C.butyricum產氫之影響………………………36
    4-2無pH控制之批次厭氧醱酵……………………………...37
    4-3控制pH值之批次厭氧醱酵……………………………...38
    4-3-1 pH值對菌體生長的影響………………………………..41
    4-3-2 ORP與產氫之關係………………………………….…..42
    4-3-3 代謝酸與產氫的影響……………………………….…...45
    4-3  連續式饋料厭氧產氫醱酵…………………………..……47
    第五章 結論…………………………………………..………...49
    參考文獻………………………………………………..………...51
    Reference 參考文獻
    王前淇 (1995), “ ORP對固定化污泥反應槽操作之影響”, 國立清華大學化學工程研究所碩士論文
    白明德, “厭氧生物產氫機制與操作策略之研究”, 成功大學研究所碩士論文, 1999, 台南。
    許駿發, “工業技術人才培訓計畫講義─高溫丁醇發之理論與應用”, 經濟部工業局, 1998, 台北。
    Allan E. K., “Distribution and Activity of Microorganisms in Lakes;Effect of Physical Processes. “,Ford Y. E. eds. , pp.47-68, Aquatic Microbiology., Blackwell Scientific Publications, Inc., 1993, Boston.
    Brosseau, J. D. and Zajic, J. E.,” Hydrogen gas production with Citrobacter intermedius and Clostridium pasteurianum.”, J. Chem. Tech. Biotechnol., 32 496, 1982.

    Charpentier, J., Martin, G., Wacheux, H. and Gilles, P., (1998), “ ORP regulation and activated sludge:15 years of experience.” ,Wat. Sci. Technol., 38(3):197-208
    Gest, H. and Peck, H. D., Jr., “A study of the hydrogenlyase reaction with systems derived from normal and anaerobic coli-aerogenes bacteria.”, J. Bacteriol., 70, 326, 1955
    Gerhard G., “ Bacterial Metabolism .“ , Spring-Verlag New York, pp. 208-282, 1986.
    Gray, C. T. and Gest, H., “Biological formation of moleculat hydrogen.”, Science, 148, 186, 1965
    Heydrickx M., Vos De P., Thibau B., Stevens P. and Ley De J. ,” Effect of Various External Factors on the Fermentative Production of Hydrogen Gas from Glucose by Clostridium butyricum Strains in Batch Culture .“ , System. Appl. Microbiol., Vol. 9 pp. 163-168, 1987.
    Heydrickx M., Vos De P., Vancanneyt M. and Ley De J.,“ The fermentation of Glycerol by Clostridium butyricum LMG 1212t2 and 1213t1 and C. pasteurianum LMG 3285.”, Appl. Microbiol. Biotechnol., Vol. 34, pp. 637-642, 1991.
    Johns, A. T., “The mechanism of propionic acid formation by Veillonella gazogenes.”, J. Gen. Microbiol., 5, 326, 1951.
    Johns, A. T. and Barker, H. A., “Methane formation, fermentation of ethanol in the absence of CO2 by methanobacillus omelianskii.”, J. bacterial., 80, 837, 1960.
    Joseph S. T. and Eva R. K ., “ Intracellular Conditions Required for Initiation of Solvent Production by Clostridium acetobutylicum.”, Appl. and Environ. Microbiol., Vol.52, No.1, pp.86-91,1986.
    Jun M. Y., Kim S. K. and Kim S.Y. ,“ Riboflavin-Sensitized Phoooxidation of Ascorbic Acid;Kinetics and Amino Acid Effects .”, Food Chemistry, Vol. 53, No. 4, pp.397-403, 1995.
    Kataoka N., Miya A. and Kiriyama K., “ Studieson Hydrogen Production by Continuous Culture System of Hydrogen-Producing Anaerobic Bacteria .“, Wat. Sci. Tech., Vol. 36, pp. 41-47, 1997.
    Karube, I., Matsunaga, T., Tsuru, S., and Suzuki, S., “Continuous hydrogen production by immobilized whole cells of Clostridium butyricum.” , Biochim biophys. Acta, 444 , 338 , 1976.
    Karube I., Urano N., Matsunaga T. and Suzuki S., “ Hydrogen Production from Glucose by Immobilized Growing Cells of Clostridium butyricum.”, Eur. J. Appl. Microbiol. Biotechnol., Vol. 16, pp.5-9, 1982.
    Koch, F. A. and Oldham, W. K. (1985) , “ Oxidation-reduction potential- a tool for monitoring , control and optimization of biological nutrient removal systems.” , Wat. Sci. Technol., 17:259-281
    Laurence G., Christian C., Isabel V. and Philippe S. ,“ Regulation of Metabolic Shifts in Clostridium acetobutylicum ATCC 824.” , FEMS Microbiology Reviews, Vol. 17, pp. 287-297, 1995.
    Lawier, A. , ”Walker Bill to boost hydrogen sparks democratic grumbling .”, Science, 267, 613, 1995.
    May, P. S., Blanchard, G. C., and Foley, R. T., “Biochemical hydrogen generators:18th Annual Proceedings Power Sources Conferences.”, 1964, May 19-21
    Mitsui, A. In.,“Solar-Hydrogen Energy System.”, Pergamon, Oxford and New York,p.171,1979.
    Pakes, W. C. C. and Jollyman , W . H., “The bacterial decomposition of formic acid into CO2 and H2 .”, J. Chem, Soc., 79, 386, 1901
    Pourbaix, M. (1963), “ atlas dequilibres electrochimiques.”, Gauthiers – Villars et Cie, Editeur-Imprimeur-Libraire, 55, Quai des Grands Augustin, Paris.
    Rheinheimer G., “ The Influence of Environmental Factors on the Development of Microorganisms.”, Rheinheimer G. eds., Aquatic Microbiology 4th ed., pp.111-147, Baffins Lane, 1992, England.
    R. Nandi and S. Sengupta ,“Microbial Production of Hydrogen: An Oveview.”, Rohrback, G. H., Scott, W. R., and Canfield, J. H., in proceedings of the 16th Annual Power Sources Conference, 18, 1962.
    Suzuki, S., Karube, I., Matsunga, T., and Kuriyama, S.,” Biochemical energy conversion using immobilized whole cells of Clostridium butyricum.”, Biochimie, 62, 353, 1980.
    Suzuki, S., Karube, I., and Matsunaga, T., “Application of a biochemical fuel cell to wastetaters.”, Biotechnol. Bioeng. Symp. No. 8 , 501, 1978.
    Taguchi, F., Chang, J. D., Mizukami, N., Saito-Taki, T., Hasegawa, K., and Morimoto, M., “Isolation of a hydrogen productionbacteria, Clostridium beijerinckii strain AM 21B from termites.”, Can. J. Microbiol., 39, 726, 1993.
    Taguchi , F., Hasegawa , K., Saito-Taki, T., and Hara, K.,” Simultaneous production of xylanase and hydrogen using xylan in batch culture of Clostridium sp.”, strainX53, J. Ferment. Bioeng., 81, 178, 1996
    Taguchi, F., Mizukami, N., Hasegawa, K., Hasegawa, K., and Saito-Taki, T.,” Direct conversion of cellulosic materials to hydrogen by Clostridium sp. Strain no. 2.”, Enzyme Microbiol. Technol., 17, 147, 1995.
    Taguchi, F., Mizukami, N., Saito-Taki , T., and Hasegawa , K.,”Hydrogen production from continuous fermentation of xylose during growth of Clostridium sp. Strain no. 2, Can. J. Microbiol.”, 41, 536, 1995.
    Taguchi , F., Yamada , K., Hasegawa , K., Taki-Saito, T., and Hara, K., “Continuous hydrogen production by Clostridium sp. Strain no. 2 from cellulose hydrolysate in an aqueous two-phase system.”, J. Ferment. Bioeng., 82, 80, 1996.
    Thauer, R. K., Jungermann, K., and Decker, K.,” Energy conservation in chemotrophic anaerobic bacteria, Bacteriol.” , Rev.,41, 100, 1977.
    Twarog, R. and Wolfe, R. S., “Role of butyryl phosphate in the energy metabolism of Clostridium tetanomorphum.”,J. Bacteriol., 86, 112, 1965
    Wareham, D. G., Hall, K. J. and Mavinic, D. S. (1993) , “ Real-time control of aerobic-anoxic sludge digestion using ORP.”,J. Environ. Engineer., 119(1):120
    Advisor
  • Chin-Hang Shu(徐敬衡)
  • Files
  • 89321046.pdf
  • approve in 2 years
    Date of Submission 2002-07-17

    [Back to Results | New Search]


    Browse | Search All Available ETDs

    If you have dissertation-related questions, please contact with the NCU library extension service section.
    Our service phone is (03)422-7151 Ext. 57407,E-mail is also welcomed.