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Student Number 87321038
Author Shu-Ping Yang(楊淑萍)
Author's Email Address No Public.
Statistics This thesis had been viewed 1902 times. Download 2142 times.
Department Chemical and Materials Engineering
Year 1999
Semester 2
Degree Master
Type of Document Master's Thesis
Language zh-TW.Big5 Chinese
Title 三團聯共聚物及鎂離子對微脂粒物理穩定性之影響及其機制探討
Date of Defense 2000-06-21
Page Count 103
Keyword
  • liposome
  • physical stability
  • triblock copolymer
  • Abstract Liposomes are thermodynamically unstable vesicles and have been widely utilized as a model of biomembrane and as a carrier in drug delivery systems. Hence, control and predict stability of vesicle against aggregation and fusion are essential for the various applications.
    With an attempt to study the effects of tri-block copolymer (PF-127) and Mg2+ ion on liposomes physical stability and interaction mechanisms , liposomes consisted of DMPC, vitamin E and various quantities of
    PF-127 were prepared by the probe sonication method. The physical stability of liposomes were monitored by examining the changes in size with time and hydrophilic fluroscence (5(6)-carbixyfluroscence) permeability. The interaction mechanisms between liposomes were demonstracted in terms of zeta potential and interaction potential(b2/B0).
    The results revealed that incorporation of PF-127 and Mg2+ reduced the change in size at 37℃. In addition, PF-127 and Mg2+ greatly promoted the permeability of hydrophilic fluroscence both at 22℃ and 37℃. Furthermore, as the quantities of PF-127 increased, the permeability greatly increased. From the results of DSC spectrum, PF-127 affect the phase transition temperature insignificantly, indicating that PF-127 has no great impact on the intravesicle structure packing. The dates obtained from zeta potential and ITC experiments confirmed that addition of PF-127 enhanced the repulsive potential that was mainly attributed to steric force resulted from PEO group. With regard to the effects of Mg2+ on intervesicle interaction, we concluded that in the presence of 100mM Mg2+ the dominate force between liposomes is van der Waals force. However, in the case of 500mM Mg2+ the dominate force between liposomes is shifted to hydration repulsive force.
    In conclusions, the effects of PF-127 on liposome physical stability is mainly attributed to intervesicle interaction.
    Table of Content 目錄
    中文摘要………………………………………………………………Ⅰ
    英文摘要………………………………………………………………..Ⅲ
    一、 前言………………………………………………………...1
    二、 研究原理……………………………………………………4
    2.1微脂粒簡介……………………………………………………4
    2.1.1微脂粒的組成…………………………………………..4
    2.1.2微脂粒的結構…………………………………………..8
    2.1.3微脂粒的型態…………………………………………..12
    2.2微脂粒膜的性質……………………………………………….14
    2.2.1相轉移……………………………………………….14
    2.2.2膜滲透……………………………………………….18
    2.3微脂粒的穩定性……………………………………………….21
    2.3.1物理穩定性…………………………………………..21
    2.3.2化學穩定性…………………………………………..29
    2.3.3生物穩定性…………………………………………..34
    2.4陽離子於微脂粒系統之應用….………………………….36
    2.5微脂粒的表面修飾………………………..…………………40
    2.6螢光指示劑……………………………………………………44
    三、 研究動機與目的…………………………………………..46
    四、 實驗裝置與方法…………………………………………..47
    4.1實驗藥品……………………………………………………47
    4.2實驗儀器設備……………………………………………….48
    4.3實驗系統……………………………………………………49
    4.3.1溶液的配製…………………………………………..49
    4.3.2微脂粒的製備…………………………………………..49
    4.3.3粒徑的測量…………………………………………..50
    4.3.4滲透實驗……………………………………………….51
    4.3.5界面電位的測量……………………………………...51
    4.3.6 ITC實驗……………………………………………….52
    4.3.7 DSC實驗……………………………………………….52
    五、 結果與討論……………………………………………………54
    5.1粒徑分析……………………………………………………55
    5.2滲透實驗……………………………………………………58
    5.2.1螢光指示劑的校正曲線……………………………..58
    5.2.2添加物對微脂粒滲透性之影響……………………...…58
    5.3界面電位……………………………………………………61
    5.4 ITC實驗……………………………………………………63
    5.5 DSC實驗……………………………………………………67
    六、 結論……………………………………………………...102
    七、 參考文獻…………………………………………………103
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    Advisor
  • Wen-yih Chen(陳文逸)
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    Date of Submission 2000-06-21

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