Title page for 86622005


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Student Number 86622005
Author Pin-Yeh Sung(宋平業)
Author's Email Address No Public.
Statistics This thesis had been viewed 2491 times. Download 1979 times.
Department Life Science
Year 1999
Semester 2
Degree Master
Type of Document Master's Thesis
Language zh-TW.Big5 Chinese
Title Toxicity study of Trichloroethylene and Tetrachloroethylene in CHO-K1 cells
Date of Defense 2000-07-19
Page Count 45
Keyword
  • Catalase
  • cell culture
  • GSH
  • GST
  • MN
  • tetrachloroethylene
  • trichloroethylene
  • VOC
  • Abstract The development and validation of cell culture techniques for
    estimating chlorine contained organic solvents in contaminated soil and water can facilitate the overall understanding of anamal health risk. Currently, trichloroethylene (TCE) and tetrachloroethylene (PCE) are the major environmental contaminant and an occupational concern issues because of their widespread industrial use. The objective of this study was to use the cell culture techniques (Chinese hamster ovary cells, CHO-K1) to determine the toxicity of the toxicity of trichloroethylene (TCE) and tetrachloroethylene (PCE) on the CHO-K1. The CHO-K1 cells were grown in a 60-mm petri dish with a 25-mm glass dish glued in the central area. The TCE (20-80 ul) or PCE (5-20 ul) was added to the central glass well, and let it to evaporate and dissolve in the surrounding medium in
    which cells were growing. Treatment of CHO-K1 cells with either TCE or PCE resulted in a dose and exposure inhibition of cell growth. A statistically significant increase in the frequency of MN (micronuclei test) was also observed in either TCE or PCE-treated CHO-K1 cells. Low dose of TCE (5-20 ul) or (1-5 ul) treatments showed significantly enhancement the intracellular glutathione (GSH) levels. However, the level of GSH rapidly decreased in the presence of higher dose of TCE (40-80 ul) or PCE (10-20 ul). Furthermore, the GST activity was enhanced by TCE- or PCE- treatment. Depletion of cellular GSH showed
    no effect on the sensitivity of CHO-K1 cells to TCE- or PCE- treatments. The GSH-conjugate has been proposed as a reactive intermediate, which is account for the toxicity of TCE and PCE, however the GSH-dependent activation play a minor role in the biotransformation of TCE and PCE in CHO-K1cells.
    Table of Content 目錄
    壹、 中文摘要… … … … … .… … … .. … … … … . … ..IV
    貳、 英文摘要… … … … ….. .. … … .. … … … … . … ..VI
    參、 緒論… … … … … … …… … ... … … . … … … … . 1
    肆、 材料與方法.. … … … …. … .. … . … … . … … … .11
    伍、 結果… … … … … … … … … … … … … … … … ...19
    陸、 討論… … … … … … … .. … … … … … … … … ...23
    柒、 圖表… … … … … … … … … … … … … … … … ...28
    圖一、有機溶劑對細胞生長抑制的情形… …. … … … … … … .28
    圖二、有機溶劑處理後24 小時生長觀察… …… … … … … … . 29
    圖三、細胞毒性的分析… … … … … .. . … … .. … … … . 30
    圖四、細胞內Glutathione 的分析… … … … … … … … … … 31
    圖五、經BSO 前處理後再暴露在有機溶劑的結果(一) … …..... ..32
    圖六、經BSO 前處理後再暴露在有機溶劑的結果(二) … …..... ..33
    圖七、細胞Glutathione S Transferase (GST) activity 分析…...34
    圖八、細胞內Catalase 的分析… … … … … … … … … … .35
    圖九、微核的觀察… … … . … … … … . … … … … … … 36
    圖十、酸鹼的變化現象… … . … … … … …. … … … … … .37
    捌、 參考文獻… … … … … … .. … … … … … … … . … 38
    玖、 附錄… … … … … … … .. … . … …… … … .. … ..46
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    Date of Submission 2000-07-19

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