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Student Number 86622003
Author Ying-Chung Wu(巫盈忠)
Author's Email Address No Public.
Statistics This thesis had been viewed 3149 times. Download 3874 times.
Department Life Science
Year 1999
Semester 2
Degree Master
Type of Document Master's Thesis
Language zh-TW.Big5 Chinese
Title Molecular studies of Superoxide dismutase (SOD) in soybean by a free radical initiator AAPH
Date of Defense 2000-07-17
Page Count 34
Keyword
  • active oxygen species
  • AOS
  • free radical
  • native PAGE
  • SOD
  • superoxide dismutase
  • Abstract Under environmental and oxidative stress, plants can protect themselves from cellular injury. For instance, through biochemical and physiological mechanisms. Superoxide dismutase (SOD) is one of antioxidative mechanism which can be involved in scavenging the superoxide free radicals and preventing plant cells from injury. This is a typical type of an antioxidative mechanism for defending the environmental stress.
    In this study, a water soluble free radical initiator [2,2'-azobis (2-amidinopropane) dihydrochloride ] AAPH was used as a research tool to induce stress, and to investigate the gene expression in soybean. Using cytosolic Cu/Zn sod gene probe showed AAPH can affect the sod gene expression and SOD activity in soybean leaves. Results showed the level of the sod transcripts decreased in response to AAPH, but the level of SOD activity increased. Followed the Pitcher and Zilinskas report in 1996, the cytosolic Cu/Zn sod gene and SOD were also responded to ozone stress. From this result suggests that AAPH could be as a research tool to study the environmental stress, to replace an expensive ozone facilities.
    Table of Content 1. 中文摘要……………………………………………………………Ⅱ
    2. Abstract……………………………………………………………...Ⅲ
    3. 第一章 前言…………………………………………………………1
    4. 第二章 材料與方法…………………………………………..……..7
    5. 第三章 結果………………………………………………….…….19
    6. 第四章 討論………………………………………………………..22
    7. 圖一、RNA轉印裝置………………………………………………25
    圖二、以AAPH誘導自由基產生對大豆病理變化………………26
    圖三、Cytosolic Cu/Zn sod 基因PCR產物………………….…….27
    圖四、大豆cytosolic Cu/Zn sod 基因DNA序列之分析…………28
    圖五、以DIG-Labeling合成cytosolic Cu/Zn sod探針…………….29
    圖六、以1﹪formaldehyde gel鑑定總核醣核酸抽取品質….……..30
    圖七、北方轉印法實驗結果………………………………………..31
    圖八、超氧歧化?活性測定………………………………………..32
    圖九、超氧歧化?活性電泳………………………………………..33
    8. 參考文獻……………………………………………………………34
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    Date of Submission 2000-07-17

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